Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface
Identifieur interne : 000C89 ( Main/Exploration ); précédent : 000C88; suivant : 000C90Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface
Auteurs : Thanaphum Osathanon [Thaïlande] ; Chenphop Sawangmake [Thaïlande] ; Nanticha Ruangchainicom [Thaïlande] ; Pavitra Wutikornwipak [Thaïlande] ; Panisa Kantukiti [Thaïlande] ; Nunthawan Nowwarote [Thaïlande] ; Prasit Pavasant [Thaïlande]Source :
- Journal of Oral Biology and Craniofacial Research [ 2212-4268 ] ; 2015.
Abstract
The present study investigated the surface properties and murine pre-osteoblast cell (MC3T3-E1) responses of phosphoric acid (H3PO4) treated commercially pure titanium.
Titanium discs were treated with various concentration of H3PO4 (5%, 10%, and 20%; v/v) at 90 °C for 30 min. Surface properties were evaluated by profilometer, contact angle meter, and scanning electron microscopy (SEM) with energy dispersive X-rays. MC3T3-E1 attachment and spreading were evaluated by SEM and phalloidin immunohistochemistry staining.
Surface roughness and wettability were not statistically difference among all experimental and control groups. Phosphate and oxygen were detected on H3PO4 treated surfaces. At 20 min, cell attachment was significantly higher in 10% and 20% H3PO4 treated groups compared to the control. Cells exhibited orientated-cytoskeleton fibers on 20% H3PO4 modified titanium surface. Though, there was no difference in cell spreading stage among all treatment groups.
H3PO4 treatment on titanium may influence early cell response, particularly on attachment and spreading.
Url:
DOI: 10.1016/j.jobcr.2015.12.005
PubMed: 26937362
PubMed Central: 4756070
Affiliations:
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Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Sawangmake, Chenphop" sort="Sawangmake, Chenphop" uniqKey="Sawangmake C" first="Chenphop" last="Sawangmake">Chenphop Sawangmake</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Ruangchainicom, Nanticha" sort="Ruangchainicom, Nanticha" uniqKey="Ruangchainicom N" first="Nanticha" last="Ruangchainicom">Nanticha Ruangchainicom</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Wutikornwipak, Pavitra" sort="Wutikornwipak, Pavitra" uniqKey="Wutikornwipak P" first="Pavitra" last="Wutikornwipak">Pavitra Wutikornwipak</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Kantukiti, Panisa" sort="Kantukiti, Panisa" uniqKey="Kantukiti P" first="Panisa" last="Kantukiti">Panisa Kantukiti</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Nowwarote, Nunthawan" sort="Nowwarote, Nunthawan" uniqKey="Nowwarote N" first="Nunthawan" last="Nowwarote">Nunthawan Nowwarote</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Pavasant, Prasit" sort="Pavasant, Prasit" uniqKey="Pavasant P" first="Prasit" last="Pavasant">Prasit Pavasant</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Oral Biology and Craniofacial Research</title><idno type="ISSN">2212-4268</idno><idno type="eISSN">2212-4276</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Aims</title><p>The present study investigated the surface properties and murine pre-osteoblast cell (MC3T3-E1) responses of phosphoric acid (H<sub>3</sub>PO<sub>4</sub>) treated commercially pure titanium.</p></sec><sec><title>Methods</title><p>Titanium discs were treated with various concentration of H<sub>3</sub>PO<sub>4</sub> (5%, 10%, and 20%; v/v) at 90 °C for 30 min. Surface properties were evaluated by profilometer, contact angle meter, and scanning electron microscopy (SEM) with energy dispersive X-rays. MC3T3-E1 attachment and spreading were evaluated by SEM and phalloidin immunohistochemistry staining.</p></sec><sec><title>Results</title><p>Surface roughness and wettability were not statistically difference among all experimental and control groups. Phosphate and oxygen were detected on H<sub>3</sub>PO<sub>4</sub> treated surfaces. At 20 min, cell attachment was significantly higher in 10% and 20% H<sub>3</sub>PO<sub>4</sub> treated groups compared to the control. Cells exhibited orientated-cytoskeleton fibers on 20% H<sub>3</sub>PO<sub>4</sub> modified titanium surface. Though, there was no difference in cell spreading stage among all treatment groups.</p></sec><sec><title>Conclusion</title><p>H<sub>3</sub>PO<sub>4</sub> treatment on titanium may influence early cell response, particularly on attachment and spreading.</p></sec></div></front></TEI><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Osathanon, Thanaphum" sort="Osathanon, Thanaphum" uniqKey="Osathanon T" first="Thanaphum" last="Osathanon">Thanaphum Osathanon</name></noRegion><name sortKey="Kantukiti, Panisa" sort="Kantukiti, Panisa" uniqKey="Kantukiti P" first="Panisa" last="Kantukiti">Panisa Kantukiti</name><name sortKey="Nowwarote, Nunthawan" sort="Nowwarote, Nunthawan" uniqKey="Nowwarote N" first="Nunthawan" last="Nowwarote">Nunthawan Nowwarote</name><name sortKey="Osathanon, Thanaphum" sort="Osathanon, Thanaphum" uniqKey="Osathanon T" first="Thanaphum" last="Osathanon">Thanaphum Osathanon</name><name sortKey="Pavasant, Prasit" sort="Pavasant, Prasit" uniqKey="Pavasant P" first="Prasit" last="Pavasant">Prasit Pavasant</name><name sortKey="Pavasant, Prasit" sort="Pavasant, Prasit" uniqKey="Pavasant P" first="Prasit" last="Pavasant">Prasit Pavasant</name><name sortKey="Ruangchainicom, Nanticha" sort="Ruangchainicom, Nanticha" uniqKey="Ruangchainicom N" first="Nanticha" last="Ruangchainicom">Nanticha Ruangchainicom</name><name sortKey="Sawangmake, Chenphop" sort="Sawangmake, Chenphop" uniqKey="Sawangmake C" first="Chenphop" last="Sawangmake">Chenphop Sawangmake</name><name sortKey="Sawangmake, Chenphop" sort="Sawangmake, Chenphop" uniqKey="Sawangmake C" first="Chenphop" last="Sawangmake">Chenphop Sawangmake</name><name sortKey="Wutikornwipak, Pavitra" sort="Wutikornwipak, Pavitra" uniqKey="Wutikornwipak P" first="Pavitra" last="Wutikornwipak">Pavitra Wutikornwipak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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